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Organoids and bioengineered intestinal models: Potential solutions to the Cryptosporidium culturing dilemma

Gunasekera, S., Zahedi, A.ORCID: 0000-0002-0165-3797, O’Dea, M.ORCID: 0000-0002-2757-7585, King, B., Monis, P., Thierry, B., Carr, J.M. and Ryan, U.ORCID: 0000-0003-2710-9324 (2020) Organoids and bioengineered intestinal models: Potential solutions to the Cryptosporidium culturing dilemma. Microorganisms, 8 (5). Article 715.

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Cryptosporidium is a major cause of severe diarrhea-related disease in children in developing countries, but currently no vaccine or effective treatment exists for those who are most at risk of serious illness. This is partly due to the lack of in vitro culturing methods that are able to support the entire Cryptosporidium life cycle, which has led to research in Cryptosporidium biology lagging behind other protozoan parasites. In vivo models such as gnotobiotic piglets are complex, and standard in vitro culturing methods in transformed cell lines, such as HCT-8 cells, have not been able to fully support fertilization occurring in vitro. Additionally, the Cryptosporidium life cycle has also been reported to occur in the absence of host cells. Recently developed bioengineered intestinal models, however, have shown more promising results and are able to reproduce a whole cycle of infectivity in one model system. This review evaluates the recent advances in Cryptosporidium culturing techniques and proposes future directions for research that may build upon these successes.

Item Type: Journal Article
Murdoch Affiliation(s): College of Science, Health, Engineering and Education
Antimicrobial Resistance and Infectious Disease Laboratory
Vector and Waterborne Pathogens Research Group
Publisher: MDPI
Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
United Nations SDGs: Goal 3: Good Health and Well-Being
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