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Differentiation of community-associated and livestock-associated methicillin-resistant staphylococcus aureus isolates and identification of spa types by use of PCR and high-resolution melt curve analysis

Ghorashi, S.A., Heller, J., Zhang, Q., Sahibzada, S.ORCID: 0000-0001-7362-8323 and Fenwick, B. (2020) Differentiation of community-associated and livestock-associated methicillin-resistant staphylococcus aureus isolates and identification of spa types by use of PCR and high-resolution melt curve analysis. Journal of Clinical Microbiology, 58 (5). e02088-19.

Link to Published Version: https://doi.org/10.1128/JCM.02088-19
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Abstract

Infections due to methicillin-resistant Staphylococcus aureus (MRSA) are present worldwide and represent a major public health concern. The capability of PCR followed by high-resolution melt (HRM) curve analysis for the detection of community-associated and livestock-associated MRSA strains and the identification of staphylococcal protein A (spa) locus was evaluated in 74 MRSA samples which were isolated from the environment, humans, and pigs on a single piggery. PCR-HRM curve analysis identified four spa types among MRSA samples and differentiated MRSA strains accordingly. A nonsubjective differentiation model was developed according to genetic confidence percentage values produced by tested samples, which did not require visual interpretation of HRM curve results. The test was carried out at different settings, and result data were reanalyzed and confirmed with DNA sequencing. PCR-HRM curve analysis proved to be a robust and reliable test for spa typing and can be used as a tool in epidemiological studies.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: American Society for Microbiology
Copyright: © 2020 American Society for Microbiology
United Nations SDGs: Goal 3: Good Health and Well-Being
Goal 15: Life on Land
URI: http://researchrepository.murdoch.edu.au/id/eprint/55914
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