Proteomic characterisation of lupin (Lupinus angustifolius) milk as influenced by extraction techniques, seed coat and cultivars

Al-Saedi, N., Agarwal, M.ORCID: 0000-0002-8781-3850, Ma, W.ORCID: 0000-0002-1264-866X, Islam, S. and Ren, Y. (2020) Proteomic characterisation of lupin (Lupinus angustifolius) milk as influenced by extraction techniques, seed coat and cultivars. Molecules, 25 (8). Article 1782.

Abstract

Lupin seeds are rich in proteins and other essential ingredients that can help to improve human health. The protein contents in both whole and split seeds of two lupin cultivars (Mandleup and PBA Jurien) were used to produce the lupin milk using the cheesecloth and centrifuge method. Proteins were extracted from the lupin milk using thiourea/urea solubilization. The proteins were separated by a two-dimensional polyacrylamide gel electrophoresis and then identified with mass spectrometry. A total of 230 protein spots were identified, 60 of which showed differential abundances. The cheesecloth separation showed protein extractability much better than that of the centrifuge method for both the cultivars. The results from this study could offer guidance for future comparative analysis and identification of lupin milk protein and provide effective separation technique to determine specific proteins in the cheese-making process.

Item Type:	Journal Article
Murdoch Affiliation(s):	College of Science, Health, Engineering and Education
Publisher:	MDPI
Copyright:	© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/55539

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