Catalog Home Page

Preliminary study on the differences in hydrocarbons between phosphine-susceptible and -resistant strains of Rhyzopertha dominica (Fabricius) and Tribolium castaneum (Herbst) using direct immersion solid-phase microextraction coupled with GC-MS

Alnajim, I., Agarwal, M.ORCID: 0000-0002-8781-3850, Liu, T., Li, B., Du, X. and Ren, Y. (2020) Preliminary study on the differences in hydrocarbons between phosphine-susceptible and -resistant strains of Rhyzopertha dominica (Fabricius) and Tribolium castaneum (Herbst) using direct immersion solid-phase microextraction coupled with GC-MS. Molecules, 25 (7). Article 1565.

[img]
Preview
PDF - Published Version
Download (1MB) | Preview
Free to read: https://doi.org/10.3390/molecules25071565
*No subscription required

Abstract

Phosphine resistance is a worldwide issue threatening the grain industry. The cuticles of insects are covered with a layer of lipids, which protect insect bodies from the harmful effects of pesticides. The main components of the cuticular lipids are hydrocarbon compounds. In this research, phosphine-resistant and -susceptible strains of two main stored-grain insects, T. castaneum and R. dominica, were tested to determine the possible role of their cuticular hydrocarbons in phosphine resistance. Direct immersion solid-phase microextraction followed by gas chromatography-mass spectrometry (GC-MS) was applied to extract and analyze the cuticular hydrocarbons. The results showed significant differences between the resistant and susceptible strains regarding the cuticular hydrocarbons that were investigated. The resistant insects of both species contained higher amounts than the susceptible insects for the majority of the hydrocarbons, sixteen from cuticular extraction and nineteen from the homogenized body extraction for T. castaneum and eighteen from cuticular extraction and twenty-one from the homogenized body extraction for R. dominica. 3-methylnonacosane and 2-methylheptacosane had the highest significant difference between the susceptible and resistant strains of T. castaneum from the cuticle and the homogenized body, respectively. Unknown5 from the cuticle and 3-methylhentriacontane from the homogenized body recorded the highest significant differences in R. dominica. The higher hydrocarbon content is a key factor in eliminating phosphine from entering resistant insect bodies, acting as a barrier between insects and the surrounding phosphine environment.

Item Type: Journal Article
Murdoch Affiliation: College of Science, Health, Engineering and Education
Publisher: MDPI
Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
URI: http://researchrepository.murdoch.edu.au/id/eprint/55512
Item Control Page Item Control Page

Downloads

Downloads per month over past year