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Effects of dietary chitosan and nano-chitosan loaded clinoptilolite on growth and immune responses of rainbow trout (Oncorhynchus mykiss)

Khani Oushani, A., Soltani, M., Sheikhzadeh, N., Shamsaie Mehrgan, M. and Rajabi Islami, H. (2020) Effects of dietary chitosan and nano-chitosan loaded clinoptilolite on growth and immune responses of rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 98 . pp. 210-217.

Link to Published Version: https://doi.org/10.1016/j.fsi.2020.01.016
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Abstract

In this study, rainbow trout Oncorhynchus mykiss weighing 27.75 ± 0.34 g were orally subjected to eight experimental diets each in three replicates containing varying amounts of chitosan and nano-chitosan (0.05, 0.5 and 5 g kg−1) loaded in clinoptilolite (14.28 g kg−1) for 70 days; and the growth and immune responses were evaluated. Results showed that growth parameters in fish fed diets chit + clin2, chit + clin3, nchit + clin1, nchit + clin2 and nchit + clin3 were significantly higher than in fish fed the control diet. All feeds, except chit + clin3, and nchit + clin3, significantly increased the total protein level. Feeds containing chit + clin2, nchit + clin1, and nchit + clin2 significantly elevated serum lysozyme activity compared with the control group. All treatments, except chit + clin3, and nchit + clin3 exhibited higher serum immunoglobulin (Ig) level than control one. In contrast, diet nchit + clin1 significantly unregulated the expression of Ig M gene in fish head-kidney compared to other groups. Additionally, all feeds, except clinoptilolite, and nchit + clin3, significantly improved the serum complement activity. Diets chit + clin2, nchit + clin1, and nchit + clin2 also significantly elevated antibacterial activity against Yersinia ruckeri compared with the control diet. Expression of inducible nitric oxide synthase (iNOS) gene in fish fed diets clinoptilolite, chit + clin1, chit + clin3, nchit + clin1, nchit + clin2, and nchit + clin3 was significantly higher than the control diet. All diets, except clinoptilolite, increased IL-1β gene expression compared to the control group. Present results suggest that diets supplemented with nchit + clin, especially at 0.05 g kg−1 nano-chitosan inclusion, could improve growth performance and immune parameters of rainbow trout.

Item Type: Journal Article
Murdoch Affiliation: Freshwater Fish Group & Fish Health Unit
School of Veterinary and Life Sciences
Publisher: Elsevier Ltd
Copyright: © 2020 Elsevier Ltd.
URI: http://researchrepository.murdoch.edu.au/id/eprint/54521
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