Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Investigation of interference from synthetic colloids on the performance of a canine neutrophil gelatinase‐associated lipocalin immunoassay

Davis, J.ORCID: 0000-0002-7078-1645, Rossi, G.ORCID: 0000-0003-4879-9504, Miller, D.W.ORCID: 0000-0002-4634-5819, Shiel, R.E. and Raisis, A.L. (2019) Investigation of interference from synthetic colloids on the performance of a canine neutrophil gelatinase‐associated lipocalin immunoassay. Veterinary Clinical Pathology, 48 (4). pp. 710-715.

Link to Published Version: https://doi.org/10.1111/vcp.12806
*Subscription may be required

Abstract

Background
Synthetic colloid solutions, administered by rapid infusion to volume‐depleted dogs, might be present in high concentrations in subsequent urine samples. The potential for these solutions to affect the performance of ELISA measurements due to sample matrix effects when studying kidney injury biomarkers requires investigation.

Objective
We aimed to investigate two different synthetic colloid solutions, 4% succinylated bovine gelatin (GEL) and 6% hydroxyethyl starch (HES), for potential interferences with a commercially available canine neutrophil gelatinase‐associated lipocalin (NGAL) ELISA.

Methods
Assay interference was assessed by measuring the linearity of NGAL concentrations measured using a canine NGAL ELISA after serial dilution of a canine pooled urine sample with an assay diluent, GEL, or HES.

Results
NGAL recovery from urine specimens containing up to 75% HES and up to 62.5% GEL was within acceptable limits (80%‐120%). NGAL recovery from the urine specimen containing 75% GEL was poor (76%). Linear regression analysis demonstrated excellent linearity under dilution when a canine urine sample was diluted with the assay diluent, GEL, or HES.

Conclusions
The presence of large amounts (>62.5%) of GEL in canine urine samples could cause negative interference in the performance of the NGAL ELISA investigated.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: American Society for Veterinary Clinical Pathology
Copyright: © 2019 American Society for Veterinary Clinical Pathology
URI: http://researchrepository.murdoch.edu.au/id/eprint/53996
Item Control Page Item Control Page