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The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth

Thomas, P., Seim, I., Jeffery, P., Gahete, M., Maugham, M., Crisp, G., Stacey, A., Shah, E., Walpole, C., Whiteside, E., Nelson, C., Herington, A., Luque, R., Veedu, R. and Chopin, L. (2019) The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth. International Journal of Oncology, 55 (6). pp. 1223-1236.

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Abstract

Recent evidence suggests that numerous long non‑coding RNAs (lncRNAs) are dysregulated in cancer, and have critical roles in tumour development and progression. The present study investigated the ghrelin receptor antisense lncRNA growth hormone secretagogue receptor opposite strand (GHSROS) in breast cancer. Reverse transcription‑quantitative polymerase chain reaction revealed that GHSROS expression was significantly upregulated in breast tumour tissues compared with normal breast tissue. Induced overexpression of GHSROS in the MDA‑MB‑231 breast cancer cell line significantly increased cell migration in vitro, without affecting cell proliferation, a finding similar to our previous study on lung cancer cell lines. Microarray analysis revealed a significant repression of a small cluster of major histocompatibility class II genes and enrichment of immune response pathways; this phenomenon may allow tumour cells to better evade the immune system. Ectopic overexpression of GHSROS in the MDA‑MB‑231 cell line significantly increased orthotopic xenograft growth in mice, suggesting that in vitro culture does not fully capture the function of this lncRNA. This study demonstrated that GHSROS may serve a relevant role in breast cancer. Further studies are warranted to explore the function and therapeutic potential of this lncRNA in breast cancer progression.

Item Type: Journal Article
Murdoch Affiliation: Centre for Comparative Genomics
Publisher: Spandidos Publications
Copyright: © 2019 Thomas et al.
United Nations SDGs: Goal 3: Good Health and Well-Being
URI: http://researchrepository.murdoch.edu.au/id/eprint/53938
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