Anti-β2GP1 antibodies have variable effects on platelet aggregation
Betts, N.A., Ahuja, K.D.K. and Adams, M.J.ORCID: 0000-0002-7743-4515
(2013)
Anti-β2GP1 antibodies have variable effects on platelet aggregation.
Pathology, 45
(2).
pp. 155-161.
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Abstract
Aim
To investigate the effects of affinity-purified rabbit anti-β2GP1, and anti-β2GP1 purified from patients with systemic lupus erythematosus (SLE), on adenosine diphosphate (ADP)-induced aggregation.
Methods
Whole blood was collected and processed to obtain platelet poor plasma (PPP) from normal controls (n = 15) and SLE patients (n = 15). Using PPP, anti-β2GP1 titres were determined using an ELISA and IgG fractions isolated using a HiTrap protein G column. Anti-β2GP1 was purified from two SLE patients using purified β2GP1 coupled to a HiTrap NHS-activated HP column.
Results
The effect of rabbit and human derived anti-β2GP1 (0–100μg/mL), on ADP (2.5, 5μM) induced platelet aggregation were investigated using light transmission aggregometry. Rabbit anti-β2GP1 significantly inhibited all parameters of 5μM ADP-induced platelet aggregation; %Max (p = 0.028), %AUC (p = 0.014) and slope (p<0.001). In contrast, anti-β2GP1 purified from SLE patients significantly enhanced the %Max (p = 0.031) and %AUC (p = 0.007) in a concentration dependent manner, but inhibited the slope (p<0.05) of 5μM ADP-induced platelet aggregation.
Conclusion
Our data suggest anti-β2GP1 purified from different species have variable effects on in vitro platelet aggregation. The disparity between rabbit and human anti-β2GP1 may be due to the heterogeneous nature of anti-β2GP1, varying avidity or different antibody binding specificities between species.
Item Type: | Journal Article |
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Publisher: | Royal College of Pathologists of Australasia |
Copyright: | © 2013 Royal College of Pathologists of Australasia |
URI: | http://researchrepository.murdoch.edu.au/id/eprint/53623 |
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