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Aspects of immunity to Mesocestoides corti in mice with reference to the use of immunomodulating agents

White, Terence Ronald (1984) Aspects of immunity to Mesocestoides corti in mice with reference to the use of immunomodulating agents. PhD thesis, Murdoch University.

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Abstract

This thesis is concerned with immunological aspects of Mesocestoides corti infections in mice. The preliminary work was concerned with the characterisation of laboratory infections with tetrathyridia of M. corti. in mice, in order to establish a standardised infection regime and provide base-line data for subsequent studies.

Resistance to M. corti was compared in six inbred strains of mice. The C57BL/6 strain showed a lower initial susceptibility to infection and this was independent of both sex and route of inoculation. Other strains showed a uniform susceptibility to infection. The higher initial resistance of C57BL/6 mice was impaired by splenectomy and irradiation indicating that it had an immunological basis.

Humoral responses to M. corti appeared to be made by both C57BL/6 and CBA/H mice. The humoral factor in immune serum retarded parasite proliferation in passive transfer experiments. Indirect evidence associated this activity with immunoglobulin. There was also evidence for the higher resistance of C57BL/6 mice having a cellular basis.

A range of immunomodulators were screened for their potential to induce resistance to M. corti. BCG and its derivative cord factor. P. acnes and lentinan all induced significant resistance in prophylactic and therapeutic regimes. The development of resistance were both time and dose dependent.

Under certain conditions prophylactic administration of BCG and P. acnes resulted in abnormally increased liver burdens. This was occasionally a feature of the therapeutic use of BCG. Some evidence was obtained that this reflected the activities of T-suppressor cells.

Immunotherapeutic resistance induced by BCG was transferred by spleen cells but not serum. This resistance developed in mice exposed to both M. corti and BCG. Spleen cells sensitised to another proliferating larval cestode Taenia crassiceps and BCG were unable to protect against challenge with M. corti. This suggested that BCG induced responses were specifically expressed to M. corti.

Some evidence was obtained that immunotherapeutic doses of P. acnes and lentinan induced resistance to M. corti in athymic mice. P. acnes substituted for BCG in an immunotherapeutic regime. In addition. These results were consistent with a hypothesis that BCG, P. acnes and lentinan activated the same effector cell population which may have been a component of the reticuloendothelial system. In intact mice, the resistance expressed by this cell population may have been specific involving bound cytophilic receptors.

Item Type: Thesis (PhD)
Murdoch Affiliation: School of Veterinary Studies
Notes: Note to the author: If you would like to make your thesis openly available on Murdoch University Library's Research Repository, please contact: repository@murdoch.edu.au. Thank you.
Supervisor(s): Thompson, Andrew
URI: http://researchrepository.murdoch.edu.au/id/eprint/53318
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