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Alternative splicing of a barley gene results in an excess-tillering and semi-dwarf mutant

Hua, W., Tan, C., Xie, J., Zhu, J., Shang, Y., Yang, J., Zhang, X-Q, Wu, X., Wang, J. and Li, C. (2019) Alternative splicing of a barley gene results in an excess-tillering and semi-dwarf mutant. Theoretical and Applied Genetics . In Press.

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Tillering and plant height are important traits determining plant architecture and grain production in cereal crops. This study identified an excess-tillering semi-dwarf mutant (htd) from an EMS-treated barley population. Genetic analysis of the F1, F2, and F2:3 populations showed that a single recessive gene controlled the excess-tillering semi-dwarf in htd. Using BSR-Seq and gene mapping, the Hvhtd gene was delimited within a 1.8 Mb interval on chromosome 2HL. Alignment of the RNA-Seq data with the functional genes in the interval identified a gene HORVU2Hr1G098820 with alternative splicing between exon2 and exon3 in the mutant, due to a G to A single-nucleotide substitution at the exon and intron junction. An independent mutant with a similar phenotype confirmed the result, with alternative splicing between exon3 and exon4. In both cases, the alternative splicing resulted in a non-functional protein. And the gene HORVU2Hr1G098820 encodes a trypsin family protein and may be involved in the IAA signaling pathway and differs from the mechanism of Green Revolution genes in the gibberellic acid metabolic pathway.

Item Type: Journal Article
Murdoch Affiliation: Western Barley Genetics Alliance
Publisher: Springer Verlag
Copyright: © 2019 Springer-Verlag GmbH Germany, part of Springer Nature
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