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166 The differential transcriptome and ontology profiles of mural and cumulus granulosa cells in stimulated human antral follicles

Kõks, S., Velthut, A., Sarapik, A., Altmäe, S., Reinmaa, E., Schalkwyk, L.C., Fernandes, C., Lad, H., Soomets, U., Jaakma, Ü.W. and Salumets, A. (2011) 166 The differential transcriptome and ontology profiles of mural and cumulus granulosa cells in stimulated human antral follicles. Reproduction, Fertility and Development, 23 (1). pp. 185-186.

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Communication between various cell types in the ovary is a prerequisite for successful folliculogenesis and ovulation. In human antral follicles, granulosa cells divide into 2 distinct cell populations: mural (enveloping the antrum, MGC) and cumulus granulosa cells (surrounding the oocyte, CGC). During infertility treatment using in vitro fertilization (IVF), granulosa cells can be retrieved during puncture of stimulated follicles offering an excellent opportunity for analysing their functional properties. The aim of this study was to compare the transcriptomes of MGC and CGC. Twenty infertile women undergoing IVF-ICSI treatment were enrolled. The MGC were obtained from follicular fluid and CGC were acquired after oocyte denudation before micromanipulation. Gene expression of both cell populations was analysed using a genome-wide transcription array. The expression profile of the 2 granulosa cell populations varied significantly: out of 28 869 transcripts, 4480 were differentially expressed (q-value < 10–4); 623 transcripts differed in their expression levels by at least 2-fold. The transcriptome of MGC showed higher expression of genes involved in immune regulation (toll-like receptors, IL18, IL17R). In CGC, pathways participating in intercellular interactions, tissue remodelling and protein degradation were more clearly distinguished (tenascin C, IGFBP5). Among the identified differentially expressed genes, several are involved in follicle development, oocyte maturation, or ovulatory processes. Our findings fit well with previously published data. The results provide a basis for future studies on intra- and intercellular signaling in the preovulatory follicle leading towards identifying methods for improving oocyte health, embryo selection, and ultimately IVF success rate.

Item Type: Journal Article
Publisher: CSIRO Publishing
Copyright: © 2019 CSIRO
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