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Use of tissue culture techniques for the improvement of Eucalyptus marginata Donn ex Sm

Bennett, Ian J. (1988) Use of tissue culture techniques for the improvement of Eucalyptus marginata Donn ex Sm. PhD thesis, Murdoch University.

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Tissue culture techniques were developed for the clonal propagation of jarrah (Eucalyptus marginata Donn ex Sm.). Surface sterilization presented no problems when callus cultures were required. Regeneration of shoots from callus from mature trees was only obtained when cultures were initiated from stamens or styles and when zeatin was included in the medium. Callus derived from stem segments did not regenerate shoots.

Shoot cultures derived from naked buds of trees. suspected of being resistant to jarrah leafminer (Perthida glyphora Common) infection, or jarrah dieback (Phytophthora cinnamomi Rands), were used to produce clonal plantlets to compare growth and resistance with seedlings. Surface sterilization of naked buds was most successful if material was collected during summer months and when zephiran was the sterilant. Growth and multiplication of shoots was possible in a medium containing BAP and NAA and although it improved after several subcultures, it remained variable between clones. Root induction was readily achieved from shoots obtained from juvenile explants but was more difficult with shoots from mature trees. These shoots required several subcultures (up to 12 months or more in culture) before roots could be induced. Some clones have 'not produced roots even after four years in culture.

Plantlets from mature trees were compared with seedlings in glasshouse and field experiments. The clones had mature leaf morphology and branching habit. did not produce lignotubers, flowered earlier than the seedlings and lacked the basal coppice growth which is typical of young jarrah plants.

Clonal plants were exposed to field infestation of leafminer. The mine size and larval survival within a clone was similar over two years, but in the second year the clones had significantly smaller mines than the ortet.

Jarrah clones and seedlings were inoculated with Phytophthora cinnamomi zoospores in vitro and in glasshouse experiments. When inoculated in vitro the clones showed a consistent response to infection. After inoculation in the glasshouse one clone gave similar results to seedlings indicating that its ortet was possibly a field escape from the pathogen. Three other clones were less affected than seedlings.

Item Type: Thesis (PhD)
Murdoch Affiliation(s): School of Biological and Environmental Sciences
Notes: Note to the author: If you would like to make your thesis openly available on Murdoch University Library's Research Repository, please contact: Thank you.
Supervisor(s): McComb, Jen
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