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Ancient bacteria show evidence of DNA repair

Johnson, S. S., Hebsgaard, M. B., Christensen, T. R., Mastepanov, M., Nielsen, R., Munch, K., Brand, T., Gilbert, M. T. P., Zuber, M. T., Bunce, M., Ronn, R., Gilichinsky, D., Froese, D. and Willerslev, E. (2007) Ancient bacteria show evidence of DNA repair. Proceedings of the National Academy of Sciences, 104 (36). pp. 14401-14405.

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Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability

Item Type: Journal Article
Murdoch Affiliation(s): School of Biological Sciences and Biotechnology
Publisher: Proceedings of the National Academy of Sciences of the United States of America
Copyright: © 2007 Johnson et al
Notes: Freely available online through the PNAS open access option
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