Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine
McGill, D., Chekmeneva, E., Lindon, J.C., Takats, Z. and Nicholson, J.K. (2019) Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine. Faraday Discussions, 218 . pp. 395-416.
*No subscription required
Abstract
Metabolite identification and annotation procedures are necessary for the discovery of biomarkers indicative of phenotypes or disease states, but these processes can be bottlenecked by the sheer complexity of biofluids containing thousands of different compounds. Here we describe low-cost novel SPE-NMR protocols utilising different cartridges and conditions, on both natural and artificial urine mixtures, which produce unique retention profiles useful for metabolic profiling. We find that different SPE methods applied to biofluids such as urine can be used to selectively retain metabolites based on compound taxonomy or other key functional groups, reducing peak overlap through concentration and fractionation of unknowns and hence promising greater control over the metabolite annotation/identification process.
Item Type: | Journal Article |
---|---|
Murdoch Affiliation(s): | Australian National Phenome Center |
Publisher: | Royal Society of Chemistry |
Copyright: | © 2019 The Royal Society of Chemistry |
URI: | http://researchrepository.murdoch.edu.au/id/eprint/50683 |
![]() |
Item Control Page |
Downloads
Downloads per month over past year