Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Immunohistochemical detection of haemoglobin subunit epsilon (HBE) in the developing mouse placenta

Al-Kinani, L.H., Coiacetto, F.ORCID: 0000-0002-9306-8017, Sharp, C.R.ORCID: 0000-0002-1797-9783, Rossi, G.ORCID: 0000-0003-4879-9504 and Greene, W.K. (2019) Immunohistochemical detection of haemoglobin subunit epsilon (HBE) in the developing mouse placenta. Journal of Cytology & Histology, 10 (3). Article 1000542.

PDF - Published Version
Download (947kB) | Preview


Introduction: Haemoglobin is a widely studied protein due to its important roles in physiology and pathology. Aberrant expression of haemoglobins, including primitive globins, have been reported in various sites and disease states and may have utility in some instances as diagnostic and/or prognostic markers. Despite this, robust detection of haemoglobin epsilon in the placenta during development by immunohistochemistry has not been well documented.

Aim: To evaluate a polyclonal antibody against human haemoglobin subunit epsilon (HBE) by immunohistochemistry during primitive erythropoiesis in the developing mouse placenta.

Methods and results: An immunohistochemistry protocol was developed using a commercially available anti-human haemoglobin subunit epsilon antibody on the mouse placenta at embryonic day 11.5. Strong and specific cytoplasmic staining was observed in primitive erythroid cells within the blood cell islands. By contrast, the placenta endothelium, mesothelium and mesoderm were all immunonegative for epsilon haemoglobin.

Conclusions: An immunohistochemistry protocol for the specific detection of epsilon haemoglobin was successfully developed using mouse placenta tissue. This assay has utility as a tool for the study of erythropoiesis during development and/or detecting the ectopic expression of epsilon globins in disease states such as cancer.

Item Type: Journal Article
Murdoch Affiliation(s): Medical, Molecular and Forensic Sciences
Veterinary Medicine
Publisher: OMICS International
Copyright: © 2019 Al-Kinani LH, et al.
Item Control Page Item Control Page


Downloads per month over past year