Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum

Jones, M.G.K.ORCID: 0000-0001-5002-0227 and Dale, P.J. (1982) Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum. Zeitschrift für Pflanzenphysiologie, 105 (3). pp. 267-274.

Abstract

Protoplasts have been isolated from suspension cultures that originated from single immature embryos of Italian ryegrass (Lolium multiflorum). Conditions required for efficient division and colony formation by these protoplasts have been investigated. Up to 50 % of the protoplasts developed walls and divided at low auxin (0.25 mg · 1-1 NAA; 2,4-D; IAA) and low cytokinin (0.25 mg · 1 -1, BAP) levels, but higher auxin levels were required for sustained cell division and colony formation. Culture of callus lines derived from protoplasts has not yet led to the regeneration of plants, although plants could be regenerated from one of the original suspension culture lines. The results are discussed with reference to the culture of other gramineous protoplasts.

Item Type:	Journal Article
Publisher:	Elsevier
Copyright:	© 1982 Gustav Fischer Verlag, Stuttgart.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/49850

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