Modulation and direction of the electrofusion response in plant protoplasts
Tools
Tools
Tempelaar, M.J., Duyst, A., De Vlas, S.Y., Krol, G., Symmonds, C. and Jones, M.G.K.
ORCID: 0000-0001-5002-0227
(1987)
Modulation and direction of the electrofusion response in plant protoplasts.
Plant Science, 48
(2).
pp. 99-105.
*Subscription may be required
Abstract
In experiments on electrofusion of protoplasts (from Solanum brevidens, S. tuberosum, Nicotiana plumbaginifolia) the presence of divalent cations (Ca2+) at 1 mM in the fusion medium was found to increase the yield of hybrids observed directly after fusion and decrease the duration of pulse needed for fusion. Pretreatment of protoplasts with the polyamine spermine also enhanced fusion yield, and when combined with 1 mM Ca2+ the effects were additive. The improvement in fusion yield (2–4 fold) was most marked for protoplast populations (e.g. from suspension cultured cells) that were least responsive to electrofusion in mannitol alone. Short term viability, judged from FDA fluorescence was found to be high at these increased fusion levels. Optimum fusion parameters for electrofusion thus may be determined from short term experiments. Attempts to direct to fusion response between populations of protoplasts of identical properties by pretreatment of one fusion partner with spermine were inconclusive.
| Item Type: | Journal Article |
|---|---|
| Journal or Publication Title: | Plant Science |
| Page Range: | pp. 99-105 |
| Publisher: | Elsevier |
| Copyright: | © 1987 Elsevier Ireland Ltd. |
| URI: | http://researchrepository.murdoch.edu.au/id/eprint/49702 |
 |
Item Control Page |
