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Improved isolation of dihaploid Solanum tubersoum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens

Fish, N., Karp, A. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (1987) Improved isolation of dihaploid Solanum tubersoum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens. In Vitro Cellular & Developmental Biology, 23 (8). pp. 575-580.

Link to Published Version: https://doi.org/10.1007/BF02620975
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Abstract

A modified protoplast isolation technique, applicable to a range of dihaploid Solanum tuberosum genotypes, has been developed. A combination of high calcium and high pH was used to fuse mesophyl protoplasts of dihaploid S. tuberosum (PDH40) and the diploid wild species S. brevidens. Large numbers of colonies were obtained after fusion and putative hybrids selected on the basis of phenotype from regenerated shoots. From these, 11 somatic hybrid plants have been identified by their isoenzyme patterns and morphologic characteristics. Four of these hybrids had the expected chromosome number of 48. The approach of mass culture after fusion followed by selection of hybrids from regenerated shoots and the application of somatic hybridization to potato breeding are discussed.

Item Type: Journal Article
Journal or Publication Title: In Vitro Cellular & Developmental Biology
Page Range: pp. 575-580
Publisher: Society for In Vitro Biology
Copyright: © 1987 Tissue Culture Association, Inc.
URI: http://researchrepository.murdoch.edu.au/id/eprint/49700
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