Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture

Morris, P. C., Weiler, E.W., Maddock, S.E., Jones, M.G.K.ORCID: 0000-0001-5002-0227, Lenton, J.R. and Bowles, D.J. (1988) Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture. Planta, 173 (1). pp. 110-116.

Link to Published Version: https://doi.org/10.1007/bf00394495
*Subscription may be required

Abstract

Levels of endogenous abscisic acid (ABA) in immature wheat (Triticum aestivum cv. Timmo) and barley (Hordeum vulgare cv. Golden Promise) embryos have been determined by enzyme-linked immunosorbent assay. Embryos of both cereal species showed an increase in ABA content during development on the parent plant. Immature embryos were excised and cultured in vitro on nutrient media that led to precocious germination or on media containing 9% (w/v) mannitol that maintained their developmental arrest. Barley and wheat embryos responded to these culture conditions in an identical manner with respect to changes in morphology, fresh weight, protein and lectin content. However, in complete contrast, the ABA content of barley embryos increased by an order of magnitude during culture on mannitol, whereas that of wheat embryos showed no significant change. The results are discussed within the context of the role of ABA in the regulation of embryo development.

Item Type: Journal Article
Publisher: Springer-Verlag
Copyright: © 1988 Springer-Verlag
URI: http://researchrepository.murdoch.edu.au/id/eprint/49689
Item Control Page Item Control Page