Catalog Home Page

Stable transformation of sugarbeet protoplasts by electroporation

Lindsey, K. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (1989) Stable transformation of sugarbeet protoplasts by electroporation. Plant Cell Reports, 8 (2). pp. 71-74.

Link to Published Version: https://doi.org/10.1007/BF00716841
*Subscription may be required

Abstract

Conditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L.. Highest plating efficiencies (up to 65% at day 21) were obtained if protoplasts were cultured in PGO salts (de Greef and Jacobs, 1979) supplemented with 0.1 mg/1 2,4-D, 0.01 mg/l BAP and 9% mannitol, and in 0.6% agarose rather than in liquid medium. Sensitivity to kanamycin also depended on whether protoplasts were cultured in liquid or agarose medium. Stable transformation of protoplast-derived colonies, as determined by resistance to kanamycin and Southern blot analysis, was achieved by electroporation using both rectangular and exponentially-decaying pulses.

Item Type: Journal Article
Journal or Publication Title: Plant Cell Reports
Page Range: pp. 71-74
Publisher: Springer Verlag
Copyright: © 1989 Springer-Verlag
URI: http://researchrepository.murdoch.edu.au/id/eprint/49683
Item Control Page Item Control Page