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Stable transformation of sugarbeet protoplasts by electroporation

Lindsey, K. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (1989) Stable transformation of sugarbeet protoplasts by electroporation. Plant Cell Reports, 8 (2). pp. 71-74.

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Conditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L.. Highest plating efficiencies (up to 65% at day 21) were obtained if protoplasts were cultured in PGO salts (de Greef and Jacobs, 1979) supplemented with 0.1 mg/1 2,4-D, 0.01 mg/l BAP and 9% mannitol, and in 0.6% agarose rather than in liquid medium. Sensitivity to kanamycin also depended on whether protoplasts were cultured in liquid or agarose medium. Stable transformation of protoplast-derived colonies, as determined by resistance to kanamycin and Southern blot analysis, was achieved by electroporation using both rectangular and exponentially-decaying pulses.

Item Type: Journal Article
Publisher: Springer Verlag
Copyright: © 1989 Springer-Verlag
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