Preliminary studies on optimizing protoplast culture of ryegrasses
Wenpin, C., Wu, Q-S, Liu, D. and Jones, M.G.K.ORCID: 0000-0001-5002-0227
(1992)
Preliminary studies on optimizing protoplast culture of ryegrasses.
Journal of Nanjing Agricultural University, 15
(2).
pp. 10-15.
Abstract
The suspension growth, protoplast isolation and culture of Lolium mulliflorum Lam and L.perenne-L were studied Ryegrass suspension cultures were in higher growth volume subcultured in the volume ratio of 1 ml cells to 20 ml liquid medium.The enzyme solution produccd maximum yield of the protoplasts when the proportion of cells in enzyme solution was 20%~30%. Glucose was more suitable than sucrose and mannitol in protoplast medium as carbon source or osmoticum and led to higher plating efficiency of ryegrass protoplasts.
Item Type: | Journal Article |
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Murdoch Affiliation: | Western Australian State Agricultural Biotechnology Centre |
Publisher: | Nanjing Agricultural University |
Publishers Website: | http://nauxb.njau.edu.cn/en/ |
URI: | http://researchrepository.murdoch.edu.au/id/eprint/49603 |
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