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Preliminary studies on optimizing protoplast culture of ryegrasses

Wenpin, C., Wu, Q-S, Liu, D. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (1992) Preliminary studies on optimizing protoplast culture of ryegrasses. Journal of Nanjing Agricultural University, 15 (2). pp. 10-15.


The suspension growth, protoplast isolation and culture of Lolium mulliflorum Lam and L.perenne-L were studied Ryegrass suspension cultures were in higher growth volume subcultured in the volume ratio of 1 ml cells to 20 ml liquid medium.The enzyme solution produccd maximum yield of the protoplasts when the proportion of cells in enzyme solution was 20%~30%. Glucose was more suitable than sucrose and mannitol in protoplast medium as carbon source or osmoticum and led to higher plating efficiency of ryegrass protoplasts.

Item Type: Journal Article
Murdoch Affiliation: Western Australian State Agricultural Biotechnology Centre
Publisher: Nanjing Agricultural University
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