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Evaluation of direct shoot regeneration from stem explants of potato (Solanum tuberosum L.) cv. Delaware by thidiazuron (TDZ)

Ehsanpour, A.A. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (2000) Evaluation of direct shoot regeneration from stem explants of potato (Solanum tuberosum L.) cv. Delaware by thidiazuron (TDZ). Journal of Science and Technology of Agriculture and Natural Resources, 4 (3). pp. 47-54.

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Abstract

One-, two- and three-step methods of plant regeneration from stem culture of potato cv. Delaware were tested as methods for producing plant material for gene transformation. Results showed that the one-step procedure using thidiazuron, a synthetic cytokinin, was the best for rapid plant regeneration. In this culture medium, several buds and shoots were regenerated from stem culture, while the other methods using a culture medium supplemented with combinations of GA, BAP [benzyladenine], NAA, zeatin, 2ip [isopentenyladenine] and IAA produced white and green callus. Morphology and chromosome number of all regenerated plants were similar to the original plants. Results showed that the regeneration system was suitable for cv. Delaware and that the culture conditions prevented genetic variation of the regenerated plants.

Item Type: Journal Article
Publisher: Isfahan University of Technology
URI: http://researchrepository.murdoch.edu.au/id/eprint/49483
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