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Lectin levels in tissues of cultured immature wheat embryos

Morris, P.C., Maddock, S.E., Jones, M.G.K.ORCID: 0000-0001-5002-0227 and Bowles, D.J. (1986) Lectin levels in tissues of cultured immature wheat embryos. Plant Cell Reports, 5 (6). pp. 460-463.

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Levels of wheat germ agglutinin have been determined by radioimmunoassay in tissues of immature wheat embryos cultured under different conditions in order to determine the suitability of the lectin as a marker for somatic embryogenesis. Embryos cultured on media favouring continued embryo development accumulated lectin in a similar manner to zygotic embryos in planta unless precocious germination occurred. Embryos cultured on media containing 2,4-D produced callus, and some of this developed somatic embryos. Both embryogenic and non-embryogenic callus contained WGA, that in non-embryogenic callus possibly arising from developmentally arrested root primordia.

Item Type: Journal Article
Publisher: Springer Verlag
Copyright: © Springer Verlag 1986
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