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Evaluation of the anti-endotoxin effects of polymyxin B in a feline model of endotoxemia

Sharp, C.R.ORCID: 0000-0002-1797-9783, DeClue, A.E., Haak, C.E., Honaker, A.R. and Reinero, C.R. (2010) Evaluation of the anti-endotoxin effects of polymyxin B in a feline model of endotoxemia. Journal of Feline Medicine and Surgery, 12 (4). pp. 278-285.

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Directed, effective therapies for feline sepsis are needed to reduce the high morbidity and mortality associated with this disease. We investigated the anti-endotoxin effects of polymyxin B (PMB) in a blinded, placebo controlled fashion, both ex vivo in a feline whole blood culture system and in vivo, using a low-dose endotoxin infusion in cats. Serial measures of systemic inflammation, and hemodynamic stability, were compared between groups. Ex vivo, PMB significantly decreased lipopolysaccharide-induced tumor necrosis factor (TNF) production from whole blood. PMB (1 mg/kg over 30 min) demonstrated anti-endotoxin effects in vivo, including decreased peak plasma TNF activity (P<0.001) and increased white blood cell count (P=0.019), with no adverse effects. Given the apparent safety and anti-endotoxin effects of PMB in this endotoxemia model, a carefully designed, randomized, blinded, placebo controlled clinical trial evaluating the use of PMB in naturally occurring Gram-negative feline sepsis should be considered.

Item Type: Journal Article
Publisher: Elsevier
Copyright: © 2010 ISFM and AAFP
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