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Molecular characterisation of the NAM-1 genes in bread wheat in Australia

Yang, R.ORCID: 0000-0003-2563-2015, Juhász, A., Zhang, Y., Chen, X., Zhang, Y., She, M., Zhang, J.ORCID: 0000-0002-1623-4675, Maddern, R., Edwards, I., Diepeveen, D.ORCID: 0000-0002-1535-8019, Islam, S. and Ma, W.ORCID: 0000-0002-1264-866X (2018) Molecular characterisation of the NAM-1 genes in bread wheat in Australia. Crop and Pasture Science, 69 (12). pp. 1173-1181.

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The wheat NAM-B1 and NAM-A1 genes are positively associated with grain protein content (GPC) in wheat. We conducted molecular characterisation of the NAM-1 genes in 51 Australian wheat varieties (Triticum aestivum L.), with the aim of improving GPC and nitrogen-usage efficiency in Australian wheat. In summary, the wild type NAM-B1 gene, which originated from Israel, was identified in two Australian wheat varieties. Five varieties contained a deletion allele, whereas the majority (43) harboured a non-functional NAM-B1 allele and one variety contained both functional and non-functional alleles. Twenty-six Australian wheat varieties contained the NAM-A1a haplotype, which was similar to its well-characterised homoeolog NAM-B1 wild type and associated with high GPC. The NAM-D1 gene in the 51 wheat varieties was also characterised, and no gene variation in the exon regions was noted; only two single-nucleotide polymorphisms in introns 1 and 2 were found among the 51 varieties.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: CSIRO Publishing
Copyright: © 2018 CSIRO
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