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Molecular markers for tracking variation in lipoxygenase activity in wheat breeding

Geng, H., Zhang, Y., He, Z., Zhang, L., Appels, R., Qu, Y. and Xia, X. (2011) Molecular markers for tracking variation in lipoxygenase activity in wheat breeding. Molecular Breeding, 28 (1). pp. 117-126.

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Lipoxygenase (LOX) activity is an important factor determining the color of flour and end-use products of wheat. In the present study, quantitative trait loci (QTL) for LOX activity in common wheat were mapped using 71 doubled haploid (DH) lines derived from a Zhongyou 9507 × CA9632 cross, and SSR markers. Two QTL, QLpx. caas. 1AL and QLpx. caas-4B, were identified on chromosomes 1AL and 4B, closely associated with LOX activity. The SSR loci Xwmc312 and Xgwm251 proved to be diagnostic and explained 13.4-25.2% of the phenotypic variance for the 1AL locus and 14.3-27.0% for the 4B locus across four environments. The SSR markers Xgwm251 and Xwmc312 were validated across 198 Chinese wheat cultivars and advanced lines and showed highly significant (P < 0.01) association with LOX activity. We further established a multiplexed PCR with SSR marker combination Xwmc312/Xgwm251 to test these wheat cultivars and advanced lines. The results suggested that the marker combination Xwmc312/Xgwm251 is efficient and reliable for evaluating LOX activity and can be used in marker-assisted selection (MAS) for targeting flour color attributes to noodle and other wheat-based products.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Comparative Genomics
Publisher: Kluwer Academic Publishers
Copyright: © 2010 Springer Science+Business Media B.V.
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