Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana

Wylie, S.J.ORCID: 0000-0002-5639-7460 and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (2011) Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana. Archives of Virology, 156 (7). pp. 1251-1255.

Abstract

Deep sequencing of the polyadenylated transcriptome of a wild plant of Hardenbergia comptoniana was used to distinguish with high support complete genome sequences of two distinct isolates of Hardenbergia mosaic virus (HarMV) co-infecting it. Isolates shared 82.0% nucleotide (nt) and 86.7% amino acid (aa) identity. Isolate 57.1 (9,628 nt) had a deletion of 17 contiguous aa within the conserved core region of the coat protein (CP), while isolate 57.2 (9,675 nt) had a wild-type CP. Overall, HarMV genomes accounted for 10.69% of total transcripts sequenced, and the mutant virus genome was 23.1% more highly expressed than the wild-type genome.

Item Type:	Journal Article
Murdoch Affiliation:	Western Australian State Agricultural Biotechnology Centre School of Biological Sciences and Biotechnology
Publisher:	Springer Verlag
Copyright:	© 2011 Springer-Verlag.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/4767

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