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Development of a multiplex, PCR-based genotyping assay for African and Asian elephants for forensic purposes

Potoczniak, M.J., Chermak, M., Quarino, L., Tobe, S.S.ORCID: 0000-0002-4854-6278 and Conte, J. (2019) Development of a multiplex, PCR-based genotyping assay for African and Asian elephants for forensic purposes. International Journal of Legal Medicine, 134 (1). pp. 55-62.

Link to Published Version: https://doi.org/10.1007/s00414-019-02097-y
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Abstract

Wildlife crimes and the threats they present to elephant populations raise the need to develop and implement DNA-based methodology as an aid for wildlife forensic investigations and conservation efforts. This study describes the development of a tetra-nucleotide repeat STR multiplex, genotyping assay that will identify Asian elephant (Elephas maximus) and African elephant (Loxodonta africana) DNA. The assay targets six tetra-nucleotide STRs and two sex-typing markers simultaneously in both genera of elephants, a first for elephant genotyping assays. The developed assay has potential application in wildlife investigations to associate a biological sample to a particular individual elephant and additionally in conservation science for population management.

Item Type: Journal Article
Murdoch Affiliation: Medical, Molecular and Forensic Sciences
Publisher: Springer Verlag
Copyright: © 2019 Springer-Verlag GmbH Germany, part of Springer Nature
URI: http://researchrepository.murdoch.edu.au/id/eprint/46944
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