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Using synthetic oligonucleotides as standards in probe-based qPCR

Conte, J., Potoczniak, M.J and Tobe, S.S.ORCID: 0000-0002-4854-6278 (2018) Using synthetic oligonucleotides as standards in probe-based qPCR. BioTechniques, 64 (4). pp. 177-179.

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Real-time PCR (qPCR) is widely used in the life sciences. For quantifying DNA, a standard curve is required. Common methods for standard development are time consuming, costly, necessitate a specific skill set, and pose a contamination risk. Using a targeted synthetic oligonucleotide, such as a gBlocks® Gene Fragment, overcomes these drawbacks and provides researchers an accurate and quick solution to standard development. Here, we demonstrate that using a gBlocks fragment as a standard provides comparable sensitivity, reliability, and assay performance to a purified amplicon standard.

Item Type: Journal Article
Publisher: Informa BioSciences
Copyright: © 2018 Future Science Group
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