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Crossmatching Maternal and Fetal Blood in Sheep

Musk, G.C., Adams, M.J., Usuda, H., Kemp, M.W. and Sharp, C.R. (2018) Crossmatching Maternal and Fetal Blood in Sheep. Comparative Medicine, 68 (4). pp. 294-297.

Link to Published Version: https://doi.org/10.30802/AALAS-CM-17-000126
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Abstract

We investigated the incidence of ex vivo incompatibility between ovine maternal RBC and fetal plasma. Time-mated singleton pregnant ewes (n = 8) underwent cesarean delivery of the fetus; at the time of delivery, paired maternal and fetal blood samples were collected and subsequently separated for storage as packed RBC and fresh frozen plasma. Gel column crossmatching was performed 3 to 4 wk later. All fetus–dam crossmatches were considered major crossmatches, combining fetal (recipient) plasma with dam (donor) RBC. The plasma of 8 fetuses was cross-matched with RBC from 5 dams; all autologous controls were negative, and all but one crossmatch (1 of 40, 2.5%) were considered compatible. In addition, the plasma of 3 dams was crossmatched with RBC from 5 dams; all autologous controls were negative; however, significant incompatibility was noted. In total, 4 of 13 (30.8%) dam–dam crossmatches were considered incompatible. The results of this initial study suggest that when a single animal receives multiple blood-product transfusions, the risk of an immunologic transfusion reaction can be reduced by ensuring that the blood products are obtained from a single donor, performing a crossmatch prior to transfusion, and the use of synthetic products to increase the oxygen carrying capacity of fetal blood.

Publication Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: American Association for Laboratory Animal Science
Copyright: © 2018 American Association for Laboratory Animal Science
URI: http://researchrepository.murdoch.edu.au/id/eprint/43562
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