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Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients

Kurvits, L., Reimann, E., Kadastik-Eerme, L., Truu, L., Kingo, K., Erm, T., Kõks, S., Taba, P. and Planken, A. (2019) Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients. Frontiers in Neuroscience, 13 . Article number 13.

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Abstract

We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson’s disease (PD) patients’ peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients’ skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of Saa-1 and -2 genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. Saa-1 and -2 expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients’ serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Comparative Genomics
Publisher: Frontiers
Copyright: © 2019 Kurvits, Reimann, Kadastik-Eerme, Truu, Kingo, Erm, Kõks, Taba and Planken
United Nations SDGs: Goal 3: Good Health and Well-Being
URI: http://researchrepository.murdoch.edu.au/id/eprint/43549
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