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Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors

Chee, J., Watson, M.W.ORCID: 0000-0002-6438-9225, Chopra, A., Nguyen, B., Cook, A.M., Creaney, J., Lesterhuis, W.J., Robinson, B.W., Lee, Y.C.G., Nowak, A.K., Lake, R.A. and McDonnell, A.M. (2018) Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors. BMC Research Notes, 11 (1).

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Abstract

Objective
Pleural effusion (PE) is a common feature of malignant pleural mesothelioma. These effusions typically contain lymphocytes and malignant cells. We postulated that the PE would be a source of lymphocytes for analysis of tumor immune milieu. The aim of this study was to compare the phenotype and T cell receptor usage of pleural effusion T cells with paired concurrently drawn peripheral blood lymphocytes. We used multi-parameter flow cytometry and high-throughput T cell receptor sequencing to analyse peripheral blood and pleural effusion mononuclear cells.

Results
Both CD8+ and CD4+ T cells from effusion showed increased expression of T cell inhibitory receptors PD-1, LAG-3 and Tim-3 compared to blood. Comprehensive T cell receptor sequencing on one of the patients showed a discordant distribution of clonotypes in the antigen-experienced (PD-1+) compartment between effusion and blood, suggesting an enrichment of antigen specific clonotypes in the effusion, with potential as an immunological response biomarker.

Item Type: Journal Article
Murdoch Affiliation: Institute for Immunology and Infectious Diseases
Publisher: BioMed Central
Copyright: © The Author(s) 2018
URI: http://researchrepository.murdoch.edu.au/id/eprint/43003
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