Catalog Home Page

Distribution of Cardicola forsteri eggs in the gills of southern bluefin tuna (Thunnus maccoyii) (Castelnau, 1872

Norte dos Santos, C., Leef, M., Jones, B.ORCID: 0000-0002-0773-2007, Bott, N., Giblot-Ducray, D. and Nowak, B. (2012) Distribution of Cardicola forsteri eggs in the gills of southern bluefin tuna (Thunnus maccoyii) (Castelnau, 1872. Aquaculture, 344-349 . pp. 54-57.

Link to Published Version: https://doi.org/10.1016/j.aquaculture.2012.03.016
*Subscription may be required

Abstract

The distribution of Cardicola forsteri eggs in the gills of southern bluefin tuna (Thunnus maccoyii) was analysed. Eggs were confirmed to be C. forsteri using laser-capture microdissection and quantitative polymerase chain reaction (qPCR) analyses. Prevalence of infection was significantly higher in the second gill arch (χ2 = 6.49, P < 0.05) than in the fourth gill arch, and significantly lower in the basal region of the second gill arch (χ2 = 6.29, d.f. = 2, P < 0.05). The intensity of infection was not significantly different between the gill arches (F = 0.03, d.f. = 1, P > 0.05). Similarly, there was no significant difference in the intensity of C. forsteri eggs between the gill arches (F = 3.43, d.f. = 2, P > 0.05), or at different depth of sectioning (F = 0.08, d.f. = 1.12, P > 0.05). Results suggest that the presence of C. forsteri eggs in the gills of tuna is more likely to be detected by sampling the second gill arch. Furthermore, targeting the middle region and increasing the sectioning depth may reduce the proportion of false negatives.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: Elsevier BV
Copyright: © 2012 Elsevier B.V.
URI: http://researchrepository.murdoch.edu.au/id/eprint/42769
Item Control Page Item Control Page