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Aquatic Animal Health Subprogram: Development of improved molecular diagnostic tests for Perkinsus olseni in Australian molluscs

Gudkovs, N., Cummins, D., Jones, B.ORCID: 0000-0002-0773-2007, Colling, A., Singanallur, N.B. and Crane, M. (2016) Aquatic Animal Health Subprogram: Development of improved molecular diagnostic tests for Perkinsus olseni in Australian molluscs. CSIRO Publishing

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Abstract

Perkinsus is the widespread cause of disease and lost production in mollusc fisheries world - wide. Found mostly in temperate waters, two species are listed internationally as notifiable by the OIE and also appear on Australia's National List of Reportable Diseases of Aquatic Animals. Although Perkinsus marinus is exotic to Australia, Perkinsus olseni is enzootic and well - known as the cause of serious infections in various wild abalone populations in south - eastern Australia. The rapid identification and reliable differentiation of species is a major issue in the diagnosis and management of Perkinsosis in Australia. Traditional methods of Perkinsus diagnosis, such as histology and Ray’s thioglycollate culture, are straightforward and practical, however they lack sensitivity and fail to differentiate specific species. The molecular methods currently recommended by the OIE are based on conventional 1 - step PCR which is generally more labour intensive, slower and less sensitive than real - time PCR. The primary aim of this project was to develop and validate a species - specific real - time PCR (qPCR) assay for Perkinsus olseni

Item Type: Report
Series Name: FRDC Project No. 2011/004
Publisher: CSIRO Publishing
Copyright: © 2016 Fisheries Research and Development Corporation
URI: http://researchrepository.murdoch.edu.au/id/eprint/42720
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