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Diversity of endemic rhizobia on Christmas Island: implications for agriculture following phosphate mining

De Meyer, S.E., Ruthrof, K.X.ORCID: 0000-0003-2038-2264, Edwards, T., Hopkins, A.J.M., Hardy, G.E.St.J.ORCID: 0000-0001-7419-5064, O’Hara, G. and Howieson, J. (2018) Diversity of endemic rhizobia on Christmas Island: implications for agriculture following phosphate mining. Systematic and Applied Microbiology, 41 (6). pp. 641-649.

Link to Published Version: https://doi.org/10.1016/j.syapm.2018.07.004
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Abstract

Given that phosphate supplies may diminish and become uneconomic to mine after 2020, there is a compelling need to develop alternative industries to support the population on Christmas Island. Former mine sites could be turned into productive agricultural land, however, large-scale commercial agriculture has never been attempted, and, given the uniqueness of the island, the diversity of rhizobia prior to introducing legumes needed evaluation. Therefore, 84 rhizobia isolates were obtained from nine different hosts, both crop and introduced legumes, located at seven sites across the island. Based on 16S rRNA and recA gene sequence analysis, the isolates grouped into 13 clades clustering within the genus Bradyrhizobium, Ensifer, Cupriavidus and Rhizobium. According to the sequences of their symbiosis genes nodC and nifH, the isolates were classified into 12 and 11 clades, respectively, and clustered closest to tropical or crop legume isolates. Moreover, the symbiosis gene phylogeny and Multi Locus Sequence Analysis gene phylogeny suggested vertical transmission in the alpha-rhizobia but horizontal transmission within the beta-rhizobia. Furthermore, this study provides evidence of a large diversity of endemic rhizobia associated with both crop and introduced legumes, and highlights the necessity of inoculation for common bean, chickpea and soybean on the Island.

Publication Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: Elsevier GmbH.
Copyright: © 2018 Elsevier GmbH.
URI: http://researchrepository.murdoch.edu.au/id/eprint/41743
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