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Bronchoalveolar lavage fluid cytology and airway hyper-reactivity in clinically normal horses

Cullimore, AM, Secombe, C.J., Lester, G.D. and Robertson, I.D.ORCID: 0000-0002-4255-4752 (2018) Bronchoalveolar lavage fluid cytology and airway hyper-reactivity in clinically normal horses. Australian Veterinary Journal, 96 (8). pp. 291-296.

Link to Published Version: https://doi.org/10.1111/avj.12721
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Abstract

Objective
To characterise the relationship between bronchoalveolar lavage fluid (BALF) cytology and pulmonary function testing with histamine bronchoprovocation (HBP) methods in a population of clinically normal horses.

Design
Cross‐sectional study

Methods
Clinically normal adult horses (n = 33) underwent pulmonary function testing and HBP with a commercial flowmetric plethysmography system. BAL was performed 1–5 days later. Statistical analysis was used to assess associations between BALF cell concentration, relative inflammatory cell percentages and categorisation, and airway hyper‐reactivity (AHR).

Results
AHR (PC35 ≤ 8 mg/mL) was demonstrated in 17 (52%) of the horses. Using current definitions, BALF cytology was consistent with inflammatory airway disease in 14 (42%) of the horses and 7 of those demonstrated either mastocytic and/or eosinophilic responses. There was no correlation between total inflammatory cell counts or relative percentage and AHR. No statistical association was found between BALF inflammatory cell categories and AHR.

Conclusion
A direct association between cytological evidence of airway inflammation and AHR was not identified in this population of clinically normal horses. Determining the presence and measuring inflammatory cell mediators in BALF may more accurately reflect AHR. In addition, normal values for cell proportions in BALF may vary between different populations of horses and more appropriate regional reference ranges should be established.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: Wiley-Blackwell
Copyright: © 2018 Australian Veterinary Association
URI: http://researchrepository.murdoch.edu.au/id/eprint/41662
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