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Evaluation of stable isotope 13C6-glucose on volatile organic compounds in different stages of Mediterranean fruit fly (Medfly) Ceratitis capitata (Diptera: Tephritidae)

Al-Khshemawee, H., Agarwal, M.ORCID: 0000-0002-8781-3850 and Ren, Y. (2017) Evaluation of stable isotope 13C6-glucose on volatile organic compounds in different stages of Mediterranean fruit fly (Medfly) Ceratitis capitata (Diptera: Tephritidae). Entomology, Ornithology & Herpetology: Current Research, 6 (3). Article 1000195.

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The Mediterranean fruit fly (Medfly) Ceratitis Capitata (Diptera: Tephidae), as most of the Tephritidae species, is a pest of great economic importance around the world. For Integrated Pest Management (IPM) and biological pest management purposes, this research will investigate characterization of the broadest possible range of volatile organic compounds and the possible changing trends of volatile biological emissions during development stages of insect. During the last years, many types of research have been done to understand chemical communications between pest-pest interactions and about the insect responses to specific volatile organic compounds. An early comparison of the VOCs emitted from larvae, pupae and adult was performed. Our research focuses on the comparison of volatile compounds emitted from a different stage of Medfly using stable isotope 13C6-glucose. Gas Chromatography (GC) technique coupled with Flame Ionization Detection (FID) and gas chromatography with mass spectrometry (GC-MS) for identification of VOCs was employed. Head Space-Solid Phase Micro Extraction HSSPME method with Three-phase fiber 50/30 µm divinylbenzene/carboxen/ polydimethylsiloxane (DVB/CAR/PDMS) was used. The results showed that there are different chemicals emitted in a different stage of Medfly (Larvae, pupae, and adults M/F) especially in the adult stage. GC-MS detected 27 compounds from larvae, 23 compounds from pupae and 29 compounds from adults. These different VOCs emitted in different stages of Medfly were clearly displayed, and a broad range of emitted volatile compounds was successfully described. The characterization of release patterns could be useful tool for the selection of compounds and for further investigated in biological studies to understand of the key semi-chemicals involved in medfly behaviour.

Item Type: Journal Article
Murdoch Affiliation(s): School of Veterinary and Life Sciences
Publisher: OMICS International
Copyright: © 2017 AL-Khshemawee H, et al
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