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The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses

Wylie, S.J.ORCID: 0000-0002-5639-7460 and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (2011) The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses. Archives of Virology, 156 (3). pp. 479-482.

Link to Published Version: http://dx.doi.org/10.1007/s00705-010-0845-3
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Abstract

The complete genome sequence (9,858 nucleotides) of the Passion fruit woodiness virus isolate MU-2 was determined using Illumina sequencing. The large open reading frame (ORF) encodes a polyprotein containing 3,086 amino acids, with an AUG start codon and UAA stop codon. The polyprotein yielded 11 proteins (P1, HC-Pro, P3, PIPO, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP). Putative cleavage sites between them were identified by sequence comparison to those of other known potyviruses. Accuracy of the genome sequence information was provided by 42-1691-fold sequence coverage, and viral RNA accounted for 7.38% of total polyadenylated RNA from the host plant.

Item Type: Journal Article
Murdoch Affiliation(s): Western Australian State Agricultural Biotechnology Centre
Publisher: Springer Verlag
Copyright: © Springer-Verlag 2010
URI: http://researchrepository.murdoch.edu.au/id/eprint/4111
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