Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

The evaluation and validation of Phadebas ® paper as a presumptive screening tool for saliva on forensic exhibits

Wornes, D.J., Speers, S.J.ORCID: 0000-0003-2027-2241 and Murakami, J.A. (2018) The evaluation and validation of Phadebas ® paper as a presumptive screening tool for saliva on forensic exhibits. Forensic Science International: Genetics, 288 . pp. 81-88.

[img]
Preview
PDF - Authors' Version
Download (704kB) | Preview
Link to Published Version: https://doi.org/10.1016/j.forsciint.2018.03.049
*Subscription may be required

Abstract

The Phadebas® Forensic Press Test is routinely used for the detection of saliva. However, assessment of the use of Phadebas® paper for this purpose has not been studied extensively. The suitability of Phadebas® paper as a presumptive screening tool for saliva on forensic exhibits, was investigated by analysing the following: (1) sensitivity, (2) specificity, (3) effects of temperature on sensitivity and specificity, (4) detection of saliva in mixed body fluid samples, and (5) influence of substrate porosity. The results of this study demonstrated that Phadebas® paper is more sensitive to α-amylase activity and less specific for saliva than previously reported. The use of an examination temperature of 37 °C had no effect on sensitivity, but increased the incidence of cross-reactivity with other forensically relevant body fluid stains. Blood, urine and vaginal secretions can inhibit the detection of α-amylase activity with Phadebas® paper in mixed stains of saliva and body fluid. Substrate porosity is a weak predictor for the time taken for a saliva stain to achieve a strong positive result on Phadebas® paper. Overall, this study demonstrated that the Phadebas® Forensic Press Test has limitations as a presumptive test for the accurate identification of saliva.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: Elsevier BV
Copyright: © 2018 Elsevier B.V.
URI: http://researchrepository.murdoch.edu.au/id/eprint/40804
Item Control Page Item Control Page

Downloads

Downloads per month over past year