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Forensic techniques for the isolation of sperm cells from mixed cell fractions: A review

Blackwell, Shannen (2017) Forensic techniques for the isolation of sperm cells from mixed cell fractions: A review. Masters by Coursework thesis, Murdoch University.

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The process of separating one specific cell type from a mixed cellular sample has been utilised extensively by the scientific community. In particular, separating sperm cells from mixed cell samples, containing both male and female DNA, is typically employed within the forensic sciences. This review will examine the different sperm cell DNA isolation techniques which are used by forensic analysts, with a particular focus on differential extraction and Y-chromosome targeted DNA profiling, as these are currently the most widely used techniques. Throughout the literature, many modifications have been made to the original methods which were proposed, in an effort to increase the scientific value of both of these methods. Any method modifications which have been introduced will be reviewed, and their benefit for the forensic community will also be discussed. Although these methods have their uses, it is discovered by this review that they are both limited in their applicability, and by their overall success rates. For this reason, a more recently published technique of using immunomagnetic beads (IMBs) to separate the DNA fractions is proposed as an alternative method to the separation techniques currently employed by forensic DNA laboratories.

Item Type: Thesis (Masters by Coursework)
Murdoch Affiliation(s): School of Veterinary and Life Sciences
Supervisor(s): Chapman, Brendan
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