Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Development of Cell-Specific aptamers: Recent advances and insight into the selection procedures

Rahimizadeh, K., Al Shamaileh, H., Fratini, M., Chakravarthy, M., Stephen, M., Shigdar, S. and Veedu, R. (2017) Development of Cell-Specific aptamers: Recent advances and insight into the selection procedures. Molecules, 22 (12). p. 2070.

[img]
Preview
PDF - Published Version
Download (921kB) | Preview
Free to read: https://doi.org/10.3390/molecules22122070
*No subscription required

Abstract

Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures.

Item Type: Journal Article
Murdoch Affiliation: Centre for Comparative Genomics
Publisher: MDPI
Copyright: © 2017 MDPI AG
URI: http://researchrepository.murdoch.edu.au/id/eprint/39809
Item Control Page Item Control Page

Downloads

Downloads per month over past year