Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

A rapid method for analysis of multi-OPPs residues in wheat by HS-SPME-GC-FPD/MSD

Du, X. and Ren, Y. (2014) A rapid method for analysis of multi-OPPs residues in wheat by HS-SPME-GC-FPD/MSD. In: 11th International Working Conference on Stored Product Protection, 24 - 28 November, Chiang Mai, Thailand pp. 768-780.

[img]
Preview
PDF - Published Version
Download (343kB) | Preview

Abstract

The rapid detection of pesticides residues in wheat has become a top food security priority. A solvent free headspace solid-phase microextraction (HS-SPME) has been evaluated for rapid screening of Organophosphorus pesticides (OPPs) residues in wheat with high sensitivity. The wheat sample (35 g) was placed in a 100 mL sealed amber glass vial and heated at 60°C for 45 min. The 8 OPP residues were extracted with a 50 μm/ 30μm DVB/CAR/PDMS fiber. In the HS-SPME procedures, the fiber was exposed in the headspace above sample for 45 min extraction, and then removed from the HS and injected in the GC injection port at 250°C for desorption of extracted chemicals. The multi residues were determined with a GC flame photometric detector GC-FPD and qualified by a GC mass spectrometer detector (GC-MSD). Seven fortification levels of 8 organophosphorus pesticides on wheat were analyzed. The GC responses for 50μm/30μm DVB/CAR/PDMS fiber were increased with increasing the fortification levels and there were significant (r2 > 0.985) linear regressions. The Lowest of Detections (LODs) of the multi pesticide standards were evaluated under the conditions of the validation study in a range of levels from 0 (control) to 100 ng (pesticides residue) g-1 of wheat that separated on a low-polar GC capillary column (Agilent DB-35UI). The result of HS-SPME method was compared with QuEChERS AOAC 2007.3 method.

Item Type: Conference Paper
Murdoch Affiliation(s): School of Veterinary and Life Sciences
URI: http://researchrepository.murdoch.edu.au/id/eprint/38561
Item Control Page Item Control Page

Downloads

Downloads per month over past year