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Advances in technology for detection of plant pathogenic microorganisms

O'Brien, P.A. (2003) Advances in technology for detection of plant pathogenic microorganisms. In: Phytophthora in Forests and Natural Ecosystems: 2nd International IUFRO Working Party 7.02.09 Meeting, 30 September - 5 October, 2001, Albany, Western Australia.

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DNA diagnostics are developing at a rapid pace being fuelled by the needs of the medical diagnostic and genomics industries. The genomics industry is based on high throughput analysis of genomes in the search for DNA markers linked to valuable traits. The industry requires analysis of thousands of samples and a major criterion is that the cost of analysis must be kept low. The development of high throughput facilities based on robotic workstations, solid state PCR and MALDI-TOF analysis makes this feasible with a cost of less than $1 per sample. In parallel. microarray technology has developed to the stage where it is possible to screen entire genomes for genetic defects instantaneously. This is increasingly being applied in human and veterinary medicine and in other areas such as environmental health, water analysis. certification of transgenic foodstuffs. and in the food industry where instant genotyping or pathogen profiling is apriority. It is inevitable that these technologies will impact on plant pathology diagnostics. Already in Australia, and other parts of the world we are seeing the establishment of commercial high throughput services offering pathogen profiling of soil samples using either DNA probe technology or MALDI-TOF analysis.

Item Type: Conference Item
Murdoch Affiliation(s): School of Biological Sciences and Biotechnology
Notes: In: JA McComb, GE StJ Hardy and IC Tommerup (eds), 'Phytophthora in Forests and Natural Ecosystems'. 2nd International IUFRO Working Party 7.02.09 Meeting, Albany, W. Australia 30th Sept - 5th Oct 2001
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