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Molecular markers in Australian isolates of Rhizoctonia solani

O'Brien, P.A. (1994) Molecular markers in Australian isolates of Rhizoctonia solani. Mycological Research, 98 (6). pp. 665-671.

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Isolates of Rhizctonia solani from different locations within Australia and Japan were analysed for restriction fragment length polymorphisms (RFLPs). These isolates belong to different anastomosis and pectic zymogram groups. Southern blots of restriction enzyme digested DNA were probed with a cloned 18S ribosomal RNA (rRNA) gene, or random cloned fragments of R. solani DNA. The patterns obtained with the rRNA probe revealed significant variation within some of the groups whereas other groups were more uniform. Group specific patterns could not be identified for all groups. There was less variation in the RFLP patterns when random cloned fragments of DNA were used as probes and group specific patterns could be identified for all groups.

Item Type: Journal Article
Murdoch Affiliation(s): School of Biological and Environmental Sciences
Publisher: Cambridge University Press
Copyright: © 1994, British Mycological Society.
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