Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni

Feng, Y., Yang, W., Ryan, U.ORCID: 0000-0003-2710-9324, Zhang, L., Kvac, M., Koudela, B., Modry, D., Li, N., Fayer, R. and Xiao, L. (2011) Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni. Journal of Clinical Microbiology, 49 (1). pp. 34-41.

PDF - Published Version
Download (1MB)
Free to read:
*No subscription required


Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, two of the most common gastric Cryptosporidium spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data for microsatellite and minisatellite sequences. Among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were eventually chosen. DNA sequence analyses of 27 C. muris and 17 C. andersoni DNA preparations showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all four loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Thus, an MLST technique was developed for the highresolution typing of C. muris and C. andersoni. It should be useful for the characterization of the population genetics and transmission of gastric Cryptosporidium spp.

Item Type: Journal Article
Publisher: American Society for Microbiology
Copyright: © 2011, American Society for Microbiology
Item Control Page Item Control Page


Downloads per month over past year