The Human Transcription Enhancer Factor-1, TEF-1, Can Substitute for Drosophila scalloped during Wingblade Development

Deshpande, N., Chopra, A., Rangarajan, A., Shashidhara, L.S., Rodrigues, V. and Krishna, S. (1997) The Human Transcription Enhancer Factor-1, TEF-1, Can Substitute for Drosophila scalloped during Wingblade Development. Journal of Biological Chemistry, 272 (16). pp. 10664-10668.

Abstract

The human transcription enhancer factor-1 (TEF-1) belongs to a family of evolutionarily conserved proteins that have a DNA binding TEA domain. TEF-1 shares a 98% homology with Drosophila scalloped (sd) in the DNA binding domain and a 50% similarity in the activation domain. We have expressed human TEF-1 in Drosophila under the hsp-70 promoter and find that it can substitute for Sd function. The transformants rescue the wingblade defects as well as the lethality of loss-of-function alleles. Observation of reporter activity in the imaginal wing discs of the enhancer-trap alleles suggests that TEF-1 is capable of promoting sd gene regulation. The functional capability of the TEF-1 product was assessed by comparing the extent of rescue by heat shock (hs)-TEF-1 with that of hs-sd. The finding that TEF-1 can function in vivo during wingblade development offers a potent genetic system for the analysis of its function and in the identification of the molecular partners of TEF-1.

Item Type:	Journal Article
Publisher:	American Society for Biochemistry and Molecular Biology Inc.
Copyright:	© 1997 American Society for Biochemistry and Molecular Biolog
URI:	http://researchrepository.murdoch.edu.au/id/eprint/36816

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