Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Bacteriophage therapy for the control of Vibrio harveyi in greenlip abalone (Haliotis laevigata)

Wang, Y., Barton, M., Elliott, L., Li, X., Abraham, S., O'Dea, M.ORCID: 0000-0002-2757-7585 and Munro, J. (2017) Bacteriophage therapy for the control of Vibrio harveyi in greenlip abalone (Haliotis laevigata). Aquaculture, 473 . pp. 251-258.

PDF - Authors' Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (790kB) | Preview
Link to Published Version:
*Subscription may be required


Bacteriophage therapy has demonstrated promising results towards the control of bacterial infections within the aquaculture industry as an alternative therapy to antibiotics. This current research describes the efficacy of bacteriophage therapy in controlling vibriosis within abalone (Haliotis laevigata). Two bacteriophages were isolated and used in in vitro assays to determine the effect of each specific phage on the growth of specific Vibrio harveyi isolates. To demonstrate efficacy, an in vivo bioassay was performed using abalone (H. laevigata) at a water temperature of 24 °C. Characterisation of the two isolated phages revealed they were from the family, Siphoviridae. The phages had different antimicrobial abilities towards different Vibrio harveyi isolates in the in vitro assays and the bioassay demonstrated improved survival of abalone treated with bacteriophage. The results of the bioassay showed that the negative control and phage control were statistically identical, with 100% survival, but they were different from the positive bacterial control, with a 0% survival. The bacteria + phage treated group showed increased accumulative survival of 70% compared to the positive control. This is the first study to demonstrate the efficacy of bacteriophage therapy treatment for abalone. The study demonstrates that if abalones are treated with bacteriophage shortly after Vibrio harveyi infection, the accumulative survival could be significantly increased.

Item Type: Journal Article
Murdoch Affiliation(s): School of Veterinary and Life Sciences
Publisher: Elsevier BV
Copyright: © 2017 Elsevier B.V.
Item Control Page Item Control Page


Downloads per month over past year