Volume of fluid and concentration of cations and energy substrates in the uteri of mice during early pseudopregnancy

Wales, R.G. and Edirisinghe, W.R. (1989) Volume of fluid and concentration of cations and energy substrates in the uteri of mice during early pseudopregnancy. Reproduction, Fertility and Development, 1 (2). pp. 171-178.

Abstract

Estimates of the volume of fluid and the concentration of cations in the uterus were made by flushing the uterine cavity of mice mated to vasectomized males and measuring the concentrations of sodium and potassium cations in the recovered fluid. On day 1 following mating, large volumes of fluid (>90 fiL) were found in the uterus but, from days 2 to 5, only 2-5 /iL of fluid were present. The ratio of sodium to potassium fell from 4-5:1 on day 1 to 1-8:1 on days 2 to 5 of pseudopregnancy, indicating that uterine fluid in the mouse has a high K+ content ranging from 35 mEq L-1 on day 1 to 75 mEq L_1 on day 5. Glucose, lactate and pyruvate in uterine flushings were also assayed and their concentration in uterine fluid calculated using the volumes found above. The level of all substrates was low on day 1 after mating. From day 2 onwards approximately 1 mM glucose was present in the fluids. The concentration of lactate was more variable, and peaked at 4 mM on day 2 of pseudopregnancy. In general, the concentration of pyruvate was 10% of the lactate value.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Veterinary Studies
Publisher:	CSIRO Publishing
Copyright:	© CSIRO 1989
URI:	http://researchrepository.murdoch.edu.au/id/eprint/34806

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