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Preservation of rhizobia

Hungria, M., Ardley, J., O'Hara, G.W. and Howieson, J.G. (2016) Preservation of rhizobia. In: Howieson, J.G. and Dilworth, M.J., (eds.) Working with rhizobia. Australian Centre for International Agricultural Research, Canberra, pp. 61-71.

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Experimentation with strains of rhizobia can last for many decades; hence there must be a reliable and efficient means of storing the bacteria. While many re¬positories of rhizobia have been developed since the symbiosis was scientifically understood, few remain available for exploitation. This is because strains were commonly stored on agar and remained the responsibility of an enthusiast, who may not necessarily have been replaced by his institution upon retirement. Agar slope-borne cultures have a relatively finite life. For this reason we recommend long-term preservation of valuable cultures lyophilized in glass tubes which will ensure survival over long periods of inattention.

Item Type: Book Chapter
Murdoch Affiliation(s): Centre for Rhizobium Studies
Publisher: Australian Centre for International Agricultural Research
Copyright: © Australian Centre for International Agricultural Research (ACIAR) 2016
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