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Collecting nodules for isolation of rhizobia

Howieson, J.G., Yates, R.J., Bala, A. and Hungria, M. (2016) Collecting nodules for isolation of rhizobia. In: Howieson, J.G. and Dilworth, M.J., (eds.) Working with rhizobia. Australian Centre for International Agricultural Research, Canberra, pp. 25-37.

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For the legumes widely used in commerce, rhizobial strains that are well matched to these for nitrogen fixation (i.e. highly effective at N2 fixation) are usually avail¬able from manufacturers or from gene banks. Some of these sources are listed in Table 2.1. However, new acquisitions of rhizobia may be required for many reasons, for example: to overcome poor N2 fixation from an existing symbiotic relationship as described in Case 2, Chapter 1; to select well-adapted strains for a difficult environment (e.g. acid soil); to assist in legume domestication programs; to match with sequenced legumes for genetic studies of N2 fixation (e.g. Terpolilli et al. 2008); or to undertake biodiversity studies.
If researchers feel that currently available strains may not satisfy their research program, then a broader range of rhizobium germplasm must be sought. Strains of rhizobia for many legumes have long been collected from their natural envi¬ronments for this purpose. These environments represent in situ repositories of rhizobium genetic resources (Date 1982). After the collection, isolation and eval¬uation of new strains they should be deposited in curated gene banks. The bulk of this chapter covers methods to acquire nodule bacteria from in situ sources.

Item Type: Book Chapter
Murdoch Affiliation(s): Centre for Rhizobium Studies
Publisher: Australian Centre for International Agricultural Research
Copyright: © Australian Centre for International Agricultural Research (ACIAR) 2016
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